Description
This study designed real-time PCR assays which amplify Cryptosporidium parvum sequences with multiple nucleotide differences between type 1 and type 2. The presence of single-nucleotide polymorphisms affected the amplicon melting temperature and the melting temperature of internal fluorescent probes, the basis for a type-specific diagnostic assay. By combining two pairs of PCR primers, one specific for Cryptosporidium parvum the other for Giardia lamblia, a multiplex assay for detecting both organisms was developed. Includes 17 references, table, figures.
Product Details
- Edition:
- Vol. – No.
- Published:
- 11/01/2002
- Number of Pages:
- 8
- File Size:
- 1 file , 570 KB
- Note:
- This product is unavailable in Ukraine, Russia, Belarus
