Description
Conventional cell culture methods for the detection of enteroviruses (EV) and the Hepatitis A virus (HAV) in the environment are expensive, time consuming and may not detect noncytopathogenic strains. Direct polymerase chain reaction (PCR) is a rapid, sensitive alternative but it is often complicated by inhibitory factors and small reaction volumes. An integrated cell culture/PCR (ICC/PCR) approach has been developed that provides a rapid detection method for viable and noncytopathogenic viruses in PCR inhibitory samples. The objective of this study was to develop a rapid method for the detection of viable HAV and EV in environmental samples. Optimization of ICC/PCR for cytopathogenic and noncytopathogenic viruses may lead to a timely and cost effective method for routine monitoring of environmental samples for the presence of human viruses.
Product Details
- Published:
- 01/01/1997
- ISBN(s):
- 0898679583
- Number of Pages:
- 8
- File Size:
- 1 file
- Note:
- This product is unavailable in Ukraine, Russia, Belarus
