Description
This powerpoint presentation begins by providing a brief overview of biological perchlorate reductionand molecular biology tools (MBTs) for treatment processes. Research objectives included: develop quantitative assay targetingperchlorate-reduction gene;examine relationship between perchlorateremoval and gene expression levels; and,apply qPCR and RT-qPCR assays toperchlorate treatment bioreactor. Research tasks included:development of quantitative molecular assays forperchlorate treatment; and,application of assays to a bioreactor. Conclusions indicated that: qPCR and RT-qPCR assays provide an additionalline of evidence that microbial perchlorate reductionwas occurring in the bioreactor;cld gene copy numbers generally were higherwhen perchlorate removal was higher;cld gene transcript copy numbers generally werehigher when perchlorate removal was higher; when bioreactor performance declined, genecopies and gene transcript copies also declined; and, bioreactor failure may be explained, at leastin part, by changes in the microbial community. Includes figures.
Product Details
- Edition:
- Vol. – No.
- Published:
- 11/01/2009
- Number of Pages:
- 25
- File Size:
- 1 file , 1.3 MB
- Note:
- This product is unavailable in Ukraine, Russia, Belarus
